Reverse gyrase has heat-protective DNA chaperone activity independent of supercoiling.

  title={Reverse gyrase has heat-protective DNA chaperone activity independent of supercoiling.},
  author={Martin Kampmann and Daniela Stock},
  journal={Nucleic acids research},
  volume={32 12},
Hyperthermophilic organisms must protect their constituent macromolecules from heat-induced degradation. A general mechanism for thermoprotection of DNA in active cells is unknown. We show that reverse gyrase, the only protein that is both specific and common to all hyperthermophiles, reduces the rate of double-stranded DNA breakage approximately 8-fold at 90 degrees C. This activity does not require ATP hydrolysis and is independent of the positive supercoiling activity of the enzyme. Reverse… CONTINUE READING

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