Reverse genetics in complex multigene operons by co-transformation of the plastid genome and its application to the open reading frame previously designated psbN.

@article{Krech2013ReverseGI,
  title={Reverse genetics in complex multigene operons by co-transformation of the plastid genome and its application to the open reading frame previously designated psbN.},
  author={Katharina Krech and Han-Yi Fu and Wolfram Thiele and Stephanie Ruf and Mark Aurel Sch{\"o}ttler and Ralph Bock},
  journal={The Plant journal : for cell and molecular biology},
  year={2013},
  volume={75 6},
  pages={1062-74}
}
Reverse genetics approaches have contributed enormously to the elucidation of gene functions in plastid genomes and the determination of structure-function relationships in chloroplast multiprotein complexes. Gene knock-outs are usually performed by disrupting the reading frame of interest with a selectable marker cassette. Site-directed mutagenesis is done by placing the marker into the adjacent intergenic spacer and relying on co-integration of the desired mutation by homologous recombination… CONTINUE READING

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Reverse genetics in complex multigene operons by co - transformation of the plastid genome and its application to the open reading frame previously designated psbN. Reverse genetics approaches have contributed enormously to the elucidation of gene functions in plastid genomes and the determination of structure - function relationships in chloroplast multiprotein complexes .
Reverse genetics in complex multigene operons by co - transformation of the plastid genome and its application to the open reading frame previously designated psbN. Reverse genetics approaches have contributed enormously to the elucidation of gene functions in plastid genomes and the determination of structure - function relationships in chloroplast multiprotein complexes .
Reverse genetics in complex multigene operons by co - transformation of the plastid genome and its application to the open reading frame previously designated psbN. Reverse genetics approaches have contributed enormously to the elucidation of gene functions in plastid genomes and the determination of structure - function relationships in chloroplast multiprotein complexes .
Reverse genetics in complex multigene operons by co - transformation of the plastid genome and its application to the open reading frame previously designated psbN. Reverse genetics approaches have contributed enormously to the elucidation of gene functions in plastid genomes and the determination of structure - function relationships in chloroplast multiprotein complexes .
Moreover , our data provide evidence for involvement of the psbN gene product in the biogenesis of both photosystem I and photosystem II .
Moreover , our data provide evidence for involvement of the psbN gene product in the biogenesis of both photosystem I and photosystem II .
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