Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants

@inproceedings{GonzlezBallester2011ReverseGI,
  title={Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants},
  author={David Gonz{\'a}lez-Ballester and Wirulda Pootakham and Florence Mus and Wenqiang Yang and Claudia Catalanotti and Leonardo Magneschi and Amaury de Montaigu and Jos{\'e} Javier Higuera and Matthew Prior and A. Camacho – Galv{\'a}n and Emilio Fern{\'a}ndez and Arthur R Grossman},
  booktitle={Plant Methods},
  year={2011}
}
A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based screenings that allow for identification of strains harboring the introduced marker gene within specific genes of interest. Our results highlight the strengths and limitations of two independent screens that differed in the nature of the marker DNA used (PCR-amplified fragment containing… CONTINUE READING

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