Reverse genetics analysis of poxvirus intermediate transcription factors.

@article{Warren2012ReverseGA,
  title={Reverse genetics analysis of poxvirus intermediate transcription factors.},
  author={Robin D Warren and Catherine A Cotter and Bernard Moss},
  journal={Journal of virology},
  year={2012},
  volume={86 17},
  pages={9514-9}
}
Vaccinia virus transcription is regulated in three stages. An intermediate transcription factor, comprised of virus-encoded polypeptides A8 and A23, was previously identified by in vitro analyses. To investigate its role, we engineered cells that stably expressed both subunits and complemented the replication of A8 and A23 deletion mutant viruses. Without… CONTINUE READING