Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.

@article{Kok2015ReverseGS,
  title={Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.},
  author={F. Kok and M. Shin and C-W. Ni and A. Gupta and A. Grosse and A. van Impel and B. Kirchmaier and J. Peterson-Maduro and G. Kourkoulis and I. Male and D. DeSantis and S. Sheppard-Tindell and L. Ebarasi and C. Betsholtz and S. Schulte-Merker and S. Wolfe and N. Lawson},
  journal={Developmental cell},
  year={2015},
  volume={32 1},
  pages={
          97-108
        }
}
The widespread availability of programmable site-specific nucleases now enables targeted gene disruption in the zebrafish. In this study, we applied site-specific nucleases to generate zebrafish lines bearing individual mutations in more than 20 genes. We found that mutations in only a small proportion of genes caused defects in embryogenesis. Moreover, mutants for ten different genes failed to recapitulate published Morpholino-induced phenotypes (morphants). The absence of phenotypes in mutant… Expand
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