Reverse genetic generation of recombinant Zaire Ebola viruses containing disrupted IRF-3 inhibitory domains results in attenuated virus growth in vitro and higher levels of IRF-3 activation without inhibiting viral transcription or replication.

@article{Hartman2006ReverseGG,
  title={Reverse genetic generation of recombinant Zaire Ebola viruses containing disrupted IRF-3 inhibitory domains results in attenuated virus growth in vitro and higher levels of IRF-3 activation without inhibiting viral transcription or replication.},
  author={Amy L Hartman and Jason E Dover and Jonathan S Towner and Stuart T Nichol},
  journal={Journal of virology},
  year={2006},
  volume={80 13},
  pages={6430-40}
}
The VP35 protein of Zaire Ebola virus is an essential component of the viral RNA polymerase complex and also functions to antagonize the cellular type I interferon (IFN) response by blocking activation of the transcription factor IRF-3. We previously mapped the IRF-3 inhibitory domain within the C terminus of VP35. In the present study, we show that mutations that disrupt the IRF-3 inhibitory function of VP35 do not disrupt viral transcription/replication, suggesting that the two functions of… CONTINUE READING
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Pathogenesis of Ebola and Marburg viruses, p. 109–124

  • A. L. Hartman, J. S. Towner, S. T. Nichol
  • 2005
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