Reverse Serial Analysis of Gene Expression (SAGE) Characterization of Orphan SAGE Tags from Human Embryonic Stem Cells Identifies the Presence of Novel Transcripts and Antisense Transcription of Key Pluripotency Genes

@article{Richards2006ReverseSA,
  title={Reverse Serial Analysis of Gene Expression (SAGE) Characterization of Orphan SAGE Tags from Human Embryonic Stem Cells Identifies the Presence of Novel Transcripts and Antisense Transcription of Key Pluripotency Genes},
  author={M Richards and Siew Peng Tan and Woon Khiong Chan and Ariff Bongso},
  journal={STEM CELLS},
  year={2006},
  volume={24}
}
Serial analysis of gene expression (SAGE) is a powerful technique for the analysis of gene expression. A significant portion of SAGE tags, designated as orphan tags, however, cannot be reliably assigned to known transcripts. We used an improved reverse SAGE (rSAGE) strategy to convert human embryonic stem cell (hESC)‐specific orphan SAGE tags into longer 3′ cDNAs. We show that the systematic analysis of these 3′ cDNAs permitted the discovery of hESC‐specific novel transcripts and cis‐natural… Expand
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