Reversal of the antiinflammatory effects of methotrexate by the nonselective adenosine receptor antagonists theophylline and caffeine: evidence that the antiinflammatory effects of methotrexate are mediated via multiple adenosine receptors in rat adjuvant arthritis.

@article{Montesinos2000ReversalOT,
  title={Reversal of the antiinflammatory effects of methotrexate by the nonselective adenosine receptor antagonists theophylline and caffeine: evidence that the antiinflammatory effects of methotrexate are mediated via multiple adenosine receptors in rat adjuvant arthritis.},
  author={Mar{\'i}a Carmen Montesinos and Jeanette See Leng Yap and Avani Desai and Inmaculada Posadas and Christine McCrary and Bruce N. Cronstein},
  journal={Arthritis and rheumatism},
  year={2000},
  volume={43 3},
  pages={
          656-63
        }
}
OBJECTIVE Weekly low-dose methotrexate (MTX) remains the mainstay of second-line therapy for rheumatoid arthritis (RA). We have previously reported that adenosine, acting at specific receptors on inflammatory cells, mediates the antiinflammatory effects of MTX in both in vitro and in vivo models of acute inflammation, but the mechanism by which MTX suppresses the chronic inflammation of arthritis remains controversial. The present study was undertaken to further investigate the means by which… Expand
Suppression of inflammation by low-dose methotrexate is mediated by adenosine A2A receptor but not A3 receptor activation in thioglycollate-induced peritonitis
TLDR
Low-dose, weekly methotrexate treatment increased the adenosine concentration in the peritoneal exudates of all mice studied, and reduced the leukocyte accumulation in the wild-type mice and A3 receptors knockout mice but not in the A2A receptor knockout mice, providing strong evidence that the anti-inflammatory effects of methotreysine are mediated by different receptors in different inflammatory loci. Expand
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TLDR
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TLDR
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TLDR
MTX treatment inhibits deamination of adenosine and potentiatesadenosine-induced vasodilatation, suggesting an increased extracellular formation ofAdenosine. Expand
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TLDR
It is shown that MTX and caffeine and theophylline differentially affect a motogenic T‐cell mechanism driven by endogenous thrombospondin‐1 (TSP‐1) and its receptor, low density lipoprotein receptor‐related protein 1 (LRP1). Expand
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TLDR
The latest developments in the pharmacokinetics, mechanism of action, pharmacogenetics, and toxicity of methotrexate are herein discussed. Expand
Recent insights in the pharmacological actions of methotrexate in the treatment of rheumatoid arthritis.
TLDR
Recent data supporting the methotrexate mechanisms of action are presented, which are likely to account for its anti-proliferative and immunosuppressive effects in rheumatoid arthritis (RA). Expand
Normalization of A2A and A3 adenosine receptor up-regulation in rheumatoid arthritis patients by treatment with anti-tumor necrosis factor alpha but not methotrexate.
TLDR
A(2A) and A(3) receptor up-regulation in ERA patients and in MTX-treated RA patients was associated with high levels of TNFalpha and NF-kappaB activation, which opens the possibility of exploiting their potential role in human diseases characterized by a marked inflammatory component. Expand
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