Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells

@article{Rezania2014ReversalOD,
  title={Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells},
  author={Alireza Rezania and Jennifer E. Bruin and Payal Arora and Allison Rubin and Irina Batushansky and Ali Asadi and Shannon M. O’Dwyer and Nina Quiskamp and Majid Mojibian and Tobias Albrecht and Yu Hsuan Carol Yang and James D. Johnson and Timothy J. Kieffer},
  journal={Nature Biotechnology},
  year={2014},
  volume={32},
  pages={1121-1133}
}
Transplantation of pancreatic progenitors or insulin-secreting cells derived from human embryonic stem cells (hESCs) has been proposed as a therapy for diabetes. We describe a seven-stage protocol that efficiently converts hESCs into insulin-producing cells. Stage (S) 7 cells expressed key markers of mature pancreatic beta cells, including MAFA, and displayed glucose-stimulated insulin secretion similar to that of human islets during static incubations in vitro. Additional characterization… 

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