Reversal of aberrant splicing of beta-thalassaemia allele (IVS-2-654 C-->T) by antisense RNA expression vector in cultured human erythroid cells.

Abstract

The antisense fragment targeting the aberrant splice sites of the beta-thalassaemia allele, IVS-2-654 C-->T (beta654), pretranscript was cloned into the mammalian expression vector, pcDNA3. The recombinant construct, pCMVA, was then used to repair the defective splicing of the beta654 mutant pretranscript in cultured beta654 erythroid cells by the… (More)

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@article{Gong2000ReversalOA, title={Reversal of aberrant splicing of beta-thalassaemia allele (IVS-2-654 C-->T) by antisense RNA expression vector in cultured human erythroid cells.}, author={L M Gong and Xue Fan Gu and Yu-Tsung Chen and Zhao-rui Ren and S Z Huang and Y T Zeng}, journal={British journal of haematology}, year={2000}, volume={111 1}, pages={351-8} }