Reversal of Pactamycin Inhibition of Methionyl-Puromycin Synthesis and 80 S initiation Complex Formation by a Ribosomal Joining Factor ( initiation factors / Met-tRNAf binding / Lineweaver-Burk analysis / reticulocytes )

Abstract

A crude mixture of polypeptide chain initiation factors (0.5 M KCI ribosomal wash) from reticulocyte ribosomes was fractionated by DEAE-cellulose column chromatography. Among several initiation factors obtained from the column, one factor eluting at 0.220.25 M KCl showed a remarkable ability to overcome the inhibition of Met-puromycin and 80S initiation… (More)

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