Retrovirus vector-mediated stable gene silencing in human cell.

  title={Retrovirus vector-mediated stable gene silencing in human cell.},
  author={Chang-Mei Liu and De-Pei Liu and Wen-ji Dong and Chih-Chuan Liang},
  journal={Biochemical and biophysical research communications},
  volume={313 3},
RNA interference (RNAi) is the mechanism of sequence-specific, post-transcriptional gene silencing initiated by short interfering RNAs (siRNAs) homologous to the gene being suppressed. siRNAs, which mediate sequence-specific mRNA degradation, are duplexes of about 21-23 nucleotides with 3'-overhangs synthesized in vitro or expressed by DNA-based vector. However, these systems rely on transfection for delivery and cannot generate long-term gene silencing in vivo. This obstacle may be… CONTINUE READING