Retrovirus-mediated transfer of anti-MDR1 hammerhead ribozymes into multidrug-resistant human leukemia cells: screening for effective target sites.

@article{Kobayashi1999RetrovirusmediatedTO,
  title={Retrovirus-mediated transfer of anti-MDR1 hammerhead ribozymes into multidrug-resistant human leukemia cells: screening for effective target sites.},
  author={Hiroyuki Kobayashi and Yuzuru Takemura and Feng-qiang Wang and Takashi Oka and Takao Ohnuma},
  journal={International journal of cancer},
  year={1999},
  volume={81 6},
  pages={944-50}
}
One of the underlying mechanisms of multidrug resistance (MDR) is cellular over-production of P-glycoprotein (P-gp), which acts as a drug efflux pump. P-gp is encoded by a small group of related genes termed MDR; only MDR1 is known to confer drug resistance. To overcome P-gp-mediated drug resistance, we have developed two anti-MDR1 hammerhead ribozymes driven by the beta-actin promoter. Upon transduction of the ribozymes into MDR cells, vincristine resistance was decreased. These two ribozymes… CONTINUE READING