Retrovirus infection: effect of time and target cell number.

  title={Retrovirus infection: effect of time and target cell number.},
  author={Jeffrey R. Morgan and J M Ledoux and R G Snow and Ronald G. Tompkins and Martin L. Yarmush},
  journal={Journal of virology},
  volume={69 11},
Using a model amphotropic recombinant retrovirus encoding the Escherichia coli lacZ gene and quantitative assays to measure virus infection, we have determined the effects of time and target cell number on infectivity. Infection of various numbers of NIH 3T3 fibroblasts showed that the extent of lacZ virus infection was dependent on virus concentration and independent of target cell number. These results demonstrate that multiplicity of infection is not an accurate predictor of the efficiency… CONTINUE READING