Retroviral vectors containing a variant dihydrofolate reductase gene for drug protection and in vivo selection of hematopoietic cells.

@article{Allay1998RetroviralVC,
  title={Retroviral vectors containing a variant dihydrofolate reductase gene for drug protection and in vivo selection of hematopoietic cells.},
  author={James A. Allay and Jacques Galipeau and Raymond L. Blakley and Brian P. Sorrentino},
  journal={Stem cells},
  year={1998},
  volume={16 Suppl 1},
  pages={
          223-33
        }
}
Transfer of drug resistance genes to hematopoietic cells is being studied as a means to protect against the myelosuppression associated with cancer chemotherapy and as a strategy for the in vivo selection and amplification of genetically modified cells. The goal of this study was to test if retroviral-mediated gene transfer of a dihydrofolate reductase (DHFR) variant (L22Y) could be used for in vivo selection of transduced myeloid cells and to determine what proportion of transduced cells was… CONTINUE READING

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