Retroviral vector integration occurs in preferred genomic targets of human bone marrow-repopulating cells.

@article{Laufs2003RetroviralVI,
  title={Retroviral vector integration occurs in preferred genomic targets of human bone marrow-repopulating cells.},
  author={Stephanie Laufs and Bernhard Gentner and K Zsuzsanna Nagy and Anna Jauch and Axel Benner and Sonja Naundorf and Klaus Kuehlcke and Bernhard Schiedlmeier and Anthony D. Ho and Walter Jens Zeller and Stefan Fruehauf},
  journal={Blood},
  year={2003},
  volume={101 6},
  pages={2191-8}
}
Increasing use of hematopoietic stem cells for retroviral vector-mediated gene therapy and recent reports on insertional mutagenesis in mice and humans have created intense interest to characterize vector integrations on a genomic level. We studied retrovirally transduced human peripheral blood progenitor cells with bone marrow-repopulating ability in immune-deficient mice. By using a highly sensitive and specific ligation-mediated polymerase chain reaction (PCR) followed by sequencing of… CONTINUE READING