Retroviral transduction of a mutant dihydrofolate reductase-thymidylate synthase fusion gene into murine marrow cells confers resistance to both methotrexate and 5-fluorouracil.

@article{Capiaux2003RetroviralTO,
  title={Retroviral transduction of a mutant dihydrofolate reductase-thymidylate synthase fusion gene into murine marrow cells confers resistance to both methotrexate and 5-fluorouracil.},
  author={Gina M Capiaux and Tulin Budak-Alpdogan and Naoko Takebe and Philipp Mayer-Kuckuk and Debabrata Banerjee and Frank Maley and Joseph R. Bertino},
  journal={Human gene therapy},
  year={2003},
  volume={14 5},
  pages={435-46}
}
Gene transfer-based myeloprotection strategies against chemotherapy require the development of effective drug resistance genes or gene combinations. Our laboratory has previously generated drug-resistant mutants of dihydrofolate reductase (DHFR F/S) and thymidylate synthase (TS G52S) for myeloprotection against methotrexate (MTX) and 5-fluorouracil (5-FU… CONTINUE READING