Retroviral expression in embryonic stem cells and hematopoietic stem cells.

@article{Cherry2000RetroviralEI,
  title={Retroviral expression in embryonic stem cells and hematopoietic stem cells.},
  author={Sara R Cherry and Detlev Biniszkiewicz and Luk Van Parijs and David Baltimore and Rudolf Jaenisch},
  journal={Molecular and cellular biology},
  year={2000},
  volume={20 20},
  pages={7419-26}
}
Achieving long-term retroviral expression in primary cells has been problematic. De novo DNA methylation of infecting proviruses has been proposed as a major cause of this transcriptional repression. Here we report the development of a mouse stem cell virus (MSCV) long terminal repeat-based retroviral vector that is expressed in both embryonic stem (ES) cells and hematopoietic stem (HS) cells. Infected HS cells and their differentiated descendants maintained long-term and stable retroviral… CONTINUE READING

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