Retroviral Transduction of T Cells and T Cell Precursors.

Abstract

Transduction of lymphoid progenitors with retroviral or lentiviral vectors is a powerful experimental strategy to tease out the role of a gene or pathway in T cell development via gain-of-function or loss-of-function strategies. Here we discuss different approaches to use this powerful technology, and present some protocols that we use to transduce murine… (More)
DOI: 10.1007/978-1-4939-2809-5_8

Topics

Cite this paper

@article{Simmons2016RetroviralTO, title={Retroviral Transduction of T Cells and T Cell Precursors.}, author={Amie Simmons and Jos{\'e} Alberola-Ila}, journal={Methods in molecular biology}, year={2016}, volume={1323}, pages={99-108} }