Retroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences

  title={Retroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences},
  author={Richard S. Mitchell and Brett Beitzel and Astrid R. W. Schroder and Paul Shinn and Huaming Chen and Charles C. Berry and Joseph R. Ecker and Frederic D. Bushman},
  journal={PLoS Biology},
The completion of the human genome sequence has made possible genome-wide studies of retroviral DNA integration. Here we report an analysis of 3,127 integration site sequences from human cells. We compared retroviral vectors derived from human immunodeficiency virus (HIV), avian sarcoma-leukosis virus (ASLV), and murine leukemia virus (MLV). Effects of gene activity on integration targeting were assessed by transcriptional profiling of infected cells. Integration by HIV vectors, analyzed in two… 

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