Retrospective analysis of double-strand break rejoining data collected using warm-lysis PFGE protocols

@article{Ratnayake2005RetrospectiveAO,
  title={Retrospective analysis of double-strand break rejoining data collected using warm-lysis PFGE protocols},
  author={Ruwan K. Ratnayake and Vladimir A. Semenenko and Robert D. Stewart},
  journal={International Journal of Radiation Biology},
  year={2005},
  volume={81},
  pages={421 - 428}
}
Sample preparation procedures for the pulsed-field gel electrophoresis (PFGE) assay usually involve a lysis step at temperatures as high as 50°C. During this warm-lysis procedure, multiply damaged sites containing heat-labile sites (HLS) can be converted into double-strand breaks (DSB). Once formed, these DSB cannot be distinguished from the DSB formed directly by ionizing radiation. This paper develops a method to correct DSB estimates for the effects of HLS in warm-lysis protocols. A first… 
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Retrospective analysis of double-strand break rejoining data collected using warm-lysis PFGE protocols
TLDR
A method to correct DSB estimates for the effects of HLS in warm-lysis protocols is developed and can be used to characterize trends and uncertainties in DSB rejoining kinetics associated with the artefactual conversion of H LS into DSB.
A DNA Double-Strand Break Kinetic Rejoining Model Based on the Local Effect Model
TLDR
The predicted bi-exponential decay functions nicely reproduce the experimental curves of DSB rejoining over time obtained by means of gel electrophoresis elution techniques as reported by different labs, involving different cell types and a wide spectrum of radiation qualities.
Repair of Radiation-Induced Heat-Labile Sites is Independent of DNA-PKcs, XRCC1 and PARP
TLDR
The presence of heat-labile sites has a substantial impact on DSB induction and DSB rejoining rates measured by pulsed-field gel electrophoresis, and heat-Labile sites repair is independent of DNA-PKcs, XRCC1 and PARP.
DNA double strand break (DSB) induction and cell survival in iodine-enhanced computed tomography (CT).
TLDR
The good agreement between modeled and measured DSB and cell survival estimates provides some confidence that the presented model can be used to accurately assess biological dose for other concentrations of the same or different contrast agents.
Effects of indirect actions and oxygen on relative biological effectiveness: estimate of DSB induction and conversion induced by gamma rays and helium ions
TLDR
The results showed that both linear energy transfer (LET) and indirect action had a strong impact on the yields for DSB induction and conversion and it may be possible to increase the yields of DSBs in cancerous cells through DNA repair pathways, ultimately enhancing cell killing.
Effects of indirect actions and oxygen on relative biological effectiveness: estimate of DSB inductions and conversions induced by therapeutic proton beams
TLDR
The findings indicate that the overall effects of DSB induction and enzymatic DSB could intensify the tumor killing, while alleviate normal tissue damage when indirect actions are effectively interrupted.
Effects of Radiation Quality and Oxygen on Clustered DNA Lesions and Cell Death
TLDR
The Monte Carlo Damage Simulation (MCDS) is extended to account for reductions in the initial lesion yield arising from enhanced chemical repair of DNA radicals under hypoxic conditions, and the kinetic energy range and types of particles considered in the MCDS have been expanded to include charged particles up to and including 56Fe ions.
Cellular radiosensitivity: How much better do we understand it?
TLDR
This review discusses the current understanding of the impact of radiation on the cell and the organism gained from the array of past and present studies and attempts to provide an explanation for what it is that determines the response to radiation.

References

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