Retrofitting YACs for direct DNA transfer into plant cells.

@article{Adam1997RetrofittingYF,
  title={Retrofitting YACs for direct DNA transfer into plant cells.},
  author={Gerhard Adam and Jamie A Mullen and Karen L. Kindle},
  journal={The Plant journal : for cell and molecular biology},
  year={1997},
  volume={11 6},
  pages={1349-58}
}
The utility of plant YAC libraries prepared in conventional YAC vectors would be dramatically increased if these YACs could be used directly for plant transformation. A pair of vectors that allow clones from YAC libraries to be modified (retrofitted) for plant transformation by direct DNA transfer methods, such as particle bombardment or electroporation, has been developed. Modification of the YAC is achieved in two sequential yeast transformation steps by taking advantage of the homologous… CONTINUE READING