Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias

@article{Karst2018RetrievalOA,
  title={Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias},
  author={S{\o}ren Michael Karst and Morten Simonsen Dueholm and Simon Jon McIlroy and Rasmus Hansen Kirkegaard and Per Halkj{\ae}r Nielsen and Mads Albertsen},
  journal={Nature Biotechnology},
  year={2018},
  volume={36},
  pages={190-195}
}
Small subunit ribosomal RNA (SSU rRNA) genes, 16S in bacteria and 18S in eukaryotes, have been the standard phylogenetic markers used to characterize microbial diversity and evolution for decades. However, the reference databases of full-length SSU rRNA gene sequences are skewed to well-studied ecosystems and subject to primer bias and chimerism, which results in an incomplete view of the diversity present in a sample. We combine poly(A)-tailing and reverse transcription of SSU rRNA molecules… Expand
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