Retinoic Acid Receptor-y : Specific Immunodetection and Phosphorylation

  title={Retinoic Acid Receptor-y : Specific Immunodetection and Phosphorylation},
  author={C{\'e}cile Rochette-Egly and Yves Lutz and Martin Saunders and Lars Scheuer and M Gaub and Pierre Chambon},
Synthetic peptides corresponding to cDNAdeduced amino acid sequences unique to the human and mouse retinoic acid receptor yl (hRAR-yl and mRAR-yl, respectively) were used to generate antiRAR-yl antibodies . Four mAbs were selected, which were directed against peptides found in region Al (Ably(Al)), region F (Ab2y(mF) and Ab4y(hF)) and region D2 (Ab5y(D2)) . These antibodies specifically immunoprecipitated and recognized by Western blotting RAR-yl proteins in COS-1 cells transfected with… CONTINUE READING
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