Retinal repair by transplantation of photoreceptor precursors
@article{MacLaren2006RetinalRB, title={Retinal repair by transplantation of photoreceptor precursors}, author={Robert E. MacLaren and Rachael A. Pearson and A. MacNeil and Ronald H. Douglas and Thomas E. Salt and Masayuki Akimoto and Anand Swaroop and Jane C. Sowden and Robin R. Ali}, journal={Nature}, year={2006}, volume={444}, pages={203-207} }
Photoreceptor loss causes irreversible blindness in many retinal diseases. Repair of such damage by cell transplantation is one of the most feasible types of central nervous system repair; photoreceptor degeneration initially leaves the inner retinal circuitry intact and new photoreceptors need only make single, short synaptic connections to contribute to the retinotopic map. So far, brain- and retina-derived stem cells transplanted into adult retina have shown little evidence of being able to…
840 Citations
Neurobiology: Right timing for retina repair
- BiologyNature
- 2006
An experiment in mice shows that adult retina can incorporate new photoreceptor cells, provided the transplanted cells are committed rod precursors at a certain stage of development, defined by expression of transcription factor Nrl.
Restoration of vision after transplantation of photoreceptors
- BiologyNature
- 2012
Evidence of functional rod-mediated vision after photoreceptor transplantation in adult Gnat1−/− mice is provided and it is demonstrated that visual signals generated by transplanted rods are projected to higher visual areas, including V1.
Reversal of end-stage retinal degeneration and restoration of visual function by photoreceptor transplantation
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- 2013
It is shown that transplanted rod precursors can reform an anatomically distinct and appropriately polarized outer nuclear layer of photoreceptor cells, and visual function was restored in animals with zero rod function at baseline, suggesting that a cell therapy approach may reconstitute a light-sensitive cell layer de novo and repair a structurally damaged visual circuit.
Migration, integration and maturation of photoreceptor precursors following transplantation in the mouse retina.
- BiologyStem cells and development
- 2014
A comprehensive histological analysis of the 6-week period following rod transplantation in mice showed the restoration of visual function mediated by transplanted photoreceptors correlated with the later expression of rod α-transducin, achieving maximal function by 5 weeks.
Transplantation of human embryonic stem cell-derived photoreceptors restores some visual function in Crx-deficient mice.
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The Retinal Pigment Epithelium: a Convenient Source of New Photoreceptor cells?
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Recent success in restoring visual function through photoreceptor replacement in mouse models of photoreceptor degeneration intensifies the need to generate or regenerate photoreceptor cells for the…
Transplantation of photoreceptors into the degenerative retina: Current state and future perspectives
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Progenitor Cell Transplantation for Retinal Disease
- Biology, Medicine
- 2008
Research areas of ongoing interest include detailed examination of marker expression, alternate potential cell types, optimization of proliferation and differentiation, genetic modification of donor cells, elucidation of the role of stem cells in cancer, assuring cessation of proliferation following transplantation, and the use of biodegradable materials as a substrate for cell delivery.
Synaptic repair and vision restoration in advanced degenerating eyes by transplantation of retinal progenitor cells
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Subretinal transplantation of MACS purified photoreceptor precursor cells into the adult mouse retina.
- BiologyJournal of visualized experiments : JoVE
- 2014
Injection of enriched cell suspensions into the subretinal space of adult wild-type mice resulted in a 3-fold higher integration rate compared to unsorted cell suspensions, and magnetic-associated cell sorting (MACS) - enrichment of transplantable rod photoreceptor precursors isolated from the neonatal retina of Photoreceptor-specific reporter mice based on the cell surface marker CD73 is shown.
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