Retention mechanism of lactate dehydrogenase in anion-exchange chromatography.

Abstract

The anion-exchange retention mechanism of lactate dehydrogenase (LDH) isoenzymes was examined under conditions where the muscle (M) subunit was not retained by the sorbent matrix. Using the stoichiometric displacement model of retention to quantitate the number (Zn) of ionic groups on the protein that interact with the sorbent matrix, it was determined that… (More)

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Cite this paper

@article{Drager1987RetentionMO, title={Retention mechanism of lactate dehydrogenase in anion-exchange chromatography.}, author={Randy Drager and F. E. Regnier}, journal={Journal of chromatography}, year={1987}, volume={406}, pages={237-46} }