Resuscitation of Salmonella enterica serovar typhimurium and enterohemorrhagic Escherichia coli from the viable but nonculturable state by heat-stable enterobacterial autoinducer.

@article{Reissbrodt2002ResuscitationOS,
  title={Resuscitation of Salmonella enterica serovar typhimurium and enterohemorrhagic Escherichia coli from the viable but nonculturable state by heat-stable enterobacterial autoinducer.},
  author={Rolf Reissbrodt and Ilse Rien{\"a}cker and Julia M. Romanova and Primrose P E Freestone and Richard D Haigh and Mark Lyte and Helmut Tsch{\"a}pe and Peter H. Williams},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 10},
  pages={4788-94}
}
Salmonella enterica serovar Typhimurium and enterohemorrhagic Escherichia coli were stressed by prolonged incubation in water microcosms until it was no longer possible to observe colony formation when samples were plated on nonselective medium. Overnight incubation of samples in nutrient-rich broth medium supplemented with growth factors, however, allowed resuscitation of stressed and viable but nonculturable cells so that subsequent plating yielded observable colonies for significantly… CONTINUE READING