Resuscitation of Hamsters after Supercooling or Partial Crystallization at Body Temperatures Below 0° C.

  title={Resuscitation of Hamsters after Supercooling or Partial Crystallization at Body Temperatures Below 0° C.},
  author={A. U. Smith and James E. Lovelock and Alan Sterling Parkes},
A VARIETY of cells and tissues of mammals survive for long periods at the temperature of ‘dry ice’ − 79° C., when frozen in media containing glycerol1. At the other extreme, Andjus's work on the whole animal shows that, by special methods of cooling and rewarming, rats can be revived from deep body temperatures of about + 0.5° C.2. We are now attempting to close the gap between these two lines of work (a) by increasing the size and complexity of the isolated tissue cooled to − 79° C., as, for… 
Frostbite, Hypothermia, and Resuscitation after Freezing
If a similar area of skin of any of these mammals were frozen while still in its normal position on the body of the living animal, it would almost certainly be severely damaged and many or all of the component cells killed.
Suspended animation in cooled, supercooled and frozen rats
  • R. Andjus
  • Biology
    The Journal of physiology
  • 1955
It is shown that it is possible to reanimate the adult rat after cooling to much lower body temperatures and in spite of prolonged respiratory and cardiac arrest and the effects of repeated coolings to zero.
A simple method for reanimating ice‐cold rats and mice
It was clear, therefore, that local heating of the heart was neither necessary nor desirable in the partially frozen hamster with arrested respiration and circulation, and the question then arose whether to restore heart beats and circulation before rewarming the whole body.
  • A. Smith
  • Medicine
    Annals of the New York Academy of Sciences
  • 1959
There is no evidence that any human being whose internal temperature has reached freezing point has recovered, and the question is whether a high blood alcohol level favors survival from severe hypothermia, or whether the intoxicated merely are more liable to expose themselves.
Subfreezing Body Temperatures in Dormant Ground Squirrels
Investigators have demonstrated that the body temperature of hibernating mammals while in the dormant state is usually maintained a few degrees C. above the environmental temperature if the latter is above freezing, however, if the surrounding temperature is lowered to freezing or below, the torpid animal may react to this low temperature by awakening.
Respiratory gas exchange. Experiments in rats induced into cardiac arrest by various procedures.
The purpose of this investigation was to compare the effects of cold alone, asphyxia alone, and the combination of cold and asphyxi upon the respiratory gas exchange during the induction of hypothermia with cardiac arrest.
The Effects on Foetal Development of Freezing Pregnant Hamsters (Mesocricetus auratus)
It has been known for many years that chilling at sensitive stages of embryonic or larval life profoundly affects the development of cold-blooded animals, and Drosophila and other insects cooled as larvae show bizarre characteristics.
Cortical granules in hamster eggs.


Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures
It would appear that rapidity of freezing is less important than the avoidance of surface effects, and Revival is far better when semen is frozen in bulk than when minimal amounts in capillary tubes are used.