Results of the first external quality assessment scheme (EQA) for isolation and analysis of circulating tumour DNA (ctDNA).

@article{Haselmann2018ResultsOT,
  title={Results of the first external quality assessment scheme (EQA) for isolation and analysis of circulating tumour DNA (ctDNA).},
  author={Verena Haselmann and Parviz Ahmad-Nejad and Wolf Jochen Geilenkeuser and Angelika Duda and Merle Gabor and Romy Eichner and Simon J. Patton and Michael Neumaier},
  journal={Clinical chemistry and laboratory medicine},
  year={2018},
  volume={56 2},
  pages={220-228}
}
BACKGROUND Circulating tumour DNA (ctDNA) is considered to have a high potential for future management of malignancies. This pilot external quality assessment (EQA) scheme aimed to address issues of analytical quality in this new area of laboratory diagnostics. METHODS The EQA scheme consisted of three 2-mL EDTA-plasma samples spiked with fragmented genomic DNA with a mutant allele frequency ranging from 0% to 10% dedicated to the analysis of nine known sequence variations in KRAS codon 12/13… CONTINUE READING
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