Restructuring of wall-bound xyloglucan by transglycosylation in living plant cells.

@article{Thompson2001RestructuringOW,
  title={Restructuring of wall-bound xyloglucan by transglycosylation in living plant cells.},
  author={J. E. Thompson and Stephen C Fry},
  journal={The Plant journal : for cell and molecular biology},
  year={2001},
  volume={26 1},
  pages={
          23-34
        }
}
  • J. Thompson, S. Fry
  • Published 1 April 2001
  • Biology, Medicine
  • The Plant journal : for cell and molecular biology
Xyloglucan endotransglycosylases (XETs) cleave and then re-join xyloglucan chains and may thus contribute to both wall-assembly and wall-loosening. The present experiments demonstrate the simultaneous occurrence in vivo of two types of interpolymeric transglycosylation: "integrational" (in which a newly secreted xyloglucan reacts with a previously wall-bound one) and "restructuring" (in which one previously wall-bound xyloglucan reacts with another). Xyloglucans synthesised by cultured rose… Expand
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TLDR
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TLDR
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TLDR
Methods for extracting transglycanases from plant tissues and assaying them in vitro, either quantitatively in solution assays or by high-throughput dot-blot screens are presented. Expand
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Widespread occurrence of a covalent linkage between xyloglucan and acidic polysaccharides in suspension-cultured angiosperm cells.
TLDR
Alkali-stable, anionic complexes of xyloglucan are widespread in the cell walls of angiosperms, including gramineous monocots. Expand
Xyloglucan endotransglucosylase activity loosens a plant cell wall.
TLDR
Fluorescent xyloglucan endotransglucosylase (XET) assays demonstrate that exogeneous XTH can act on isolated onion epidermis cell walls, and provides evidence that XTHs can act as cell wall-loosening enzymes. Expand
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References

SHOWING 1-10 OF 44 REFERENCES
Xyloglucan undergoes interpolymeric transglycosylation during binding to the plant cell wall in vivo: evidence from 13C/3H dual labelling and isopycnic centrifugation in caesium trifluoroacetate.
TLDR
It is concluded that XET-catalysed interpolymeric transglycosylation accompanies, and probably causes, the integration of newly secreted xyloglucan into the cell-wall architecture. Expand
Endotransglycosylation of xyloglucans in plant cell suspension cultures.
TLDR
The results provide the first evidence that polymeric xyloglucans are subject in vivo to cleavage followed by transfer of the cut end on the other xylglucan-related molecules, and may be relevant to the understanding of plant cell growth. Expand
Trimming and solubilization of xyloglucan after deposition in the walls of cultured rose cells
TLDR
A pulse-chase technique involving the in vivo feeding of L-[1-3H]arabinose to suspension-cultured rose [Rosa) cells at 4 d and 9 d after subculture was used to create a population of [ 3H]xyloglucan molecules and to follow their subsequent fate. Expand
Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants.
TLDR
It is proposed that XET is responsible for cutting and rejoining intermicrofibrillar xyloglucan chains and that it thus causes the wall-loosening required for plant cell expansion. Expand
Ten isoenzymes of xyloglucan endotransglycosylase from plant cell walls select and cleave the donor substrate stochastically.
TLDR
The hypothesis that different XET isoenzymes are adapted to produce longer or shorter products such as might favour either the efficient integration of new xyloglucan into the cell wall or the re-structuring of old xylglucan within an expanding wall is not supported. Expand
Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule.
TLDR
It is concluded that endo-xyloglucan transferase functions as a reconnecting enzyme for xylglucans and is involved in the interweaving or reconstruction of cell wall matrix, which is responsible for chemical creepage that leads to morphological changes in the cell wall. Expand
In Vivo Colocalization of Xyloglucan Endotransglycosylase Activity and Its Donor Substrate in the Elongation Zone of Arabidopsis Roots
TLDR
A characteristic distribution pattern was found in Arabidopsis and tobacco roots: in both species, fluorescence was most prominent in the cell elongation zone of the root. Expand
Differences in catalytic properties between native isoenzymes of xyloglucan endotransglycosylase (XET).
TLDR
It is suggested that this difference is related to the major roles of the XETs in these two tissues: integration of new xyloglucan into the walls of the densely cytoplasmic cauliflower florets, and re-structuring of existing wall material in the rapidly vacuolating bean shoots. Expand
The Arabidopsis TCH4 Xyloglucan Endotransglycosylase (Substrate Specificity, pH Optimum, and Cold Tolerance)
TLDR
It is indicated that TCH4 is specific for xyloglucan, but that the molecular mass and the fucosyl content of the substrates influence enzymatic reaction rates. Expand
Pea Xyloglucan and Cellulose: VI. Xyloglucan-Cellulose Interactions in Vitro and in Vivo.
TLDR
The small size of primary wall microfibrils in higher plants may well be due to the binding of xyloglucan to cellulose during synthesis which prevents fasciation of small fibrils into larger bundles. Expand
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