Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii.

@article{Pedersen1996RestrictionFL,
  title={Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogen Vibrio ordalii.},
  author={Karen Margrethe Pedersen and S Koblavi and Tarja Tiainen and P. A-D Grimont},
  journal={Epidemiology and infection},
  year={1996},
  volume={117 2},
  pages={385-91}
}
A total of 32 Vibrio ordalii strains were studied for their plasmid content and shown to carry a plasmid of approximately 32 kb. This plasmid was subsequently subjected to restriction fragment length polymorphism (RFLP) studies. Using Hind III, three different restriction patterns were identified while BamH I cleaved the plasmid into a single linear fragment. The results suggest that the 32 kb plasmid is highly conserved but that some variation in restriction pattern occurs. The same set of… CONTINUE READING

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