Restriction fragment length polymorphism in the exon 2 of the BoLA-DRB3 gene in Chinese Holstein of the south China

Abstract

The genetic diversity of the exon2 of BoLA-DRB3 (BoLA-DRB3.2) in Chinese Holstein cattle of the south China was investigated by hemi-nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Six, four and eleven RFLP patterns were found after digestion with the restriction enzymes Hae III, Bst YI and Rsa I, respectively. The DNA sequence showed and twentyfive DRB3.2 alleles. GLM model analysis indicated that lactation and calving season have positive correlation with SCC (Somatic Cell Count) (p<0.01), BoLA-DRB3.2*3, *8, *18 and *26 were associated with lower SCC (p<0.01). The present findings concluded that mastitis is a severe hinder of milk production and technology. Therefore, future re-searches should focus on associations of mastitis with BoLA haplotypes rather than single BoLA genes

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Cite this paper

@inproceedings{Wu2010RestrictionFL, title={Restriction fragment length polymorphism in the exon 2 of the BoLA-DRB3 gene in Chinese Holstein of the south China}, author={Xiu-Xiang Wu and Zhang-ping Yang and Xiaolong Wang and Yong-Jiang Mao and Shu-Chun Li and X Shi and Ying Chen}, year={2010} }