Restriction enzyme-resistant high molecular weight telomeric DNA fragments in tobacco.

  title={Restriction enzyme-resistant high molecular weight telomeric DNA fragments in tobacco.},
  author={Katsunori Suzuki and Yasuyuki Yamagiwa and Tadashi Matsui and Kazuo Yoshida},
  journal={DNA research : an international journal for rapid publication of reports on genes and genomes},
  volume={1 3},
Restriction endonuclease-resistant high-molecular-weight (HMW) DNA fragments were isolated from nuclear DNA fragments in tobacco. The size of the fragments produced by EcoRI, HindIII, AfaI, and HaeIII ranged from 20 kb to over 166 kb. The kinetics of digestion by Bal31 nuclease showed that most of the HMW fragments are chromosome ends. The consensus sequence for tobacco telomere repeats was determined to be CCCTAAA by genomic sequencing using the HMW fragments and by sequencing after cloning… CONTINUE READING
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