Restriction enzyme-mediated DNA family shuffling.


DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without primers… (More)
DOI: 10.1007/978-1-4939-1053-3_12


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