Restriction enzyme-mediated DNA family shuffling.

Abstract

DNA shuffling is an established recombinatorial method that was originally developed to increase the speed of directed evolution experiments beyond what could be accomplished using error-prone PCR alone. To achieve this, mutated copies of a protein-coding sequence are fragmented with DNase I and the fragments are then reassembled in a PCR without primers… (More)
DOI: 10.1007/978-1-4939-1053-3_12

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