Restriction enzyme fragment length polymorphisms of amplified herpes simplex virus type-1 DNA provide epidemiologic information.

@article{Haugen1993RestrictionEF,
  title={Restriction enzyme fragment length polymorphisms of amplified herpes simplex virus type-1 DNA provide epidemiologic information.},
  author={Thomas H. Haugen and B Alden and S Matthey and Daniel Nicholson},
  journal={Diagnostic microbiology and infectious disease},
  year={1993},
  volume={17 2},
  pages={
          129-33
        }
}
Human herpes simplex type 1 (HSV-1) DNA of isolates from patients in a large teaching hospital was amplified by the polymerase chain reaction (PCR). The PCR products targeted approximately 2100 nt regions of relatively low G + C content. Comparison of restriction enzyme digests of amplified DNA showed variation useful for strain differentiation. Twelve nonrelated HSV-1 were differentiated from one another. In contrast, specimens epidemiologically related in an outbreak were indistinguishable… CONTINUE READING