Restriction endonucleases from microorganisms isolated in Brazil: an isoschizomer of HaeIII from a thermophilic Bacillus sp.

Abstract

1. The isolation and characterization of a restriction endonuclease from a thermophilic strain of Bacillus is described. 2. The enzyme recognizes the palindromic sequence 5'...GGCC...3' as determined by PEI-cellulose chromatography of pancreatic DNAse and snake venom phosphodiesterase digestion products of labelled DNA fragments, analysis of restriction… (More)

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