Restriction endonuclease mapping and mutagenesis of the F sex factor replication region

@article{Manis1977RestrictionEM,
  title={Restriction endonuclease mapping and mutagenesis of the F sex factor replication region},
  author={Jack J. Manis and Bruce C. Kline},
  journal={Molecular and General Genetics MGG},
  year={1977},
  volume={152},
  pages={175-182}
}
The plasmids pSC138 and pML31 each contain the EcoRI-generated f5 replicator fragment of the conjugative plasmid F in addition to an EcoRI fragment encoding antibiotic resistance: ampicillin resistance derived from Staphylococcus aureus in pSC138 and kanamycin resistance from Escherichia coli in pML31. We have mapped one HindIII and two BamHI restriction sites in the f5 region of these plasmids and one HindIII site in the antibiotic resistance region of each plasmid. The HindIII site in the Km… CONTINUE READING
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Molecular cloning of DNA from F sex factor of Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America • 1976

Cloning, isolation, and characterization of replication regions of complex plasmid genomes.

Proceedings of the National Academy of Sciences of the United States of America • 1975

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