Restriction and modification in Bacillus subtilis Marburg 168: Target sites and effects on plasmid transformation

Abstract

The effects of the restriction system of Bacillus subtilis strain M on plasmid transformation were studied. Plasmid pHV1401 DNA prepared from B. subtilis transformed the restriction-proficient M strain 100 times more efficiently than the DNA prepared from Escherichia coli, while the two DNA preparations transformed restriction-deficient derivatives of that… (More)
DOI: 10.1007/BF00338412

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