Restriction analysis of the Streptomyces glaucescens genome by agarose gel electrophoresis

Abstract

A method for the analysis of total DNA of Streptomyces glaucescens is described. The relevant steps are (a) extraction and purification of DNA, (b) restriction of DNA samples with type II restriction enzymes, (c) one dimensional separation of restriction fragments by agarose gel electrophoresis. A typical banding pattern was obtained for each wild type… (More)
DOI: 10.1007/BF00459522

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