Restriction analysis and partial sequencing of the 16S rRNA gene as index for rapid identification of Bacillus species

@article{Vardhan2010RestrictionAA,
  title={Restriction analysis and partial sequencing of the 16S rRNA gene as index for rapid identification of Bacillus species},
  author={Shachi Vardhan and Rajeev Kaushik and Anil Kumar Saxena and Dilip Kumar Arora},
  journal={Antonie van Leeuwenhoek},
  year={2010},
  volume={99},
  pages={283-296}
}
Restriction fragment length analysis of PCR amplified 16S rDNA with AluI revealed the presence of a 265 bp fragment in all species of Bacillus with the exception of B. cereus and B. thuringiensis, which contains two restriction sites within this fragment which results in three smaller fragments totalling to 265 bp. Some distant species of Bacillus with no evidence of this fragment could be delineated into other genera based on phenotypic and genotypic parameters. BLAST search for homologous… CONTINUE READING

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