Restricted utilization of germ-line VH3 genes and short diverse third complementarity-determining regions (CDR3) in human fetal B lymphocyte immunoglobulin heavy chain rearrangements.

@article{Raaphorst1992RestrictedUO,
  title={Restricted utilization of germ-line VH3 genes and short diverse third complementarity-determining regions (CDR3) in human fetal B lymphocyte immunoglobulin heavy chain rearrangements.},
  author={Frank M. Raaphorst and Erik Timmers and M. Kenter and Maarten J D van Tol and J. P. Vossen and Ruud K. B. Schuurman},
  journal={European journal of immunology},
  year={1992},
  volume={22 1},
  pages={247-51}
}
Twenty-one independent immunoglobulin heavy chain VH3DJH rearrangements were cloned and sequenced from livers of human fetuses at 7, 13 and 18 weeks of gestation. The VH elements expressed were not somatically mutated. Eight out of the estimated 30 VH3 elements were utilized with a preference for five of them. One of these VH3 sequences, designated FL13-28, represented a thus-far unknown VH3 gene segment. From the six functional JH elements the JH3 and JH4 segments were utilized preferentially… CONTINUE READING