Restricted Presence of the Growth Hormone-Releasing Hormone Receptor to Somatotropes in Rat and Human Pituitaries

  title={Restricted Presence of the Growth Hormone-Releasing Hormone Receptor to Somatotropes in Rat and Human Pituitaries},
  author={Gérard Morel and Rosal{\'i}a Gallego and Luce Boulanger and Elena Pintos and Tom{\'a}s Garc{\'i}a-Caballero and Pierrette Gaudreau},
  pages={128 - 136}
Specific binding of growth hormone-releasing hormone (GHRH) to its plasma membrane receptor represents the first step of cellular signals leading to exocytotic GH secretion in the anterior pituitary. The GHRH receptor (GHRH-R) has been cloned and belongs to the secretin/glucagon/vasoactive intestinal peptide subfamilly of G-protein-coupled receptors. To study its characteristics in rat and human pituitaries and examine its cellular and subcellular localization, a site-directed polyclonal… 

Figures from this paper

Expression profiles of growth hormone-releasing hormone and growth hormone-releasing hormone receptor during chicken embryonic pituitary development.
The differential expressions of GHRH, GHRHR, and GH in the developing embryonic pituitaries not only imply that pituitary-derived G HRH and G HRHR may have a paracrine/autocrine role in the expansion of undifferentiated somatotroph precursor cells, but also suggest that GHR HR is likely to be involved in the somatOTroph differentiation occurring at the later developmental stages.
Cellular distribution of growth hormone-releasing hormone receptor in human reproductive system and breast and prostate cancers.
The results demonstrate the localization of GHRH-R in the reproductive system, which may mediate the direct action ofGHRH in these tissues, opening a variety of possibilities for the use of G HRH antagonists in the treatment of prostatic and mammary tumors.
The growth hormone (GH)-axis of GH receptor/binding protein gene-disrupted and metallothionein-human GH-releasing hormone transgenic mice: hypothalamic neuropeptide and pituitary receptor expression in the absence and presence of GH feedback.
It is suggested that GH regulates its own synthesis and release not only by altering expression of key hypothalamic neuropeptides but also by modulating the sensitivity of the pituitary to hypothalamic input, by regulating pituitsary receptor synthesis.
Pituitary-specific expression and Pit-1 regulation of the rat growth hormone-releasing hormone receptor gene.
The results show that the 1.6-kb promoter is sufficient to direct tissue- and cell-specific expression in vivo and is regulated bypituitary-specific transcription factor Pit-1.
Expression of genes involved in the somatotropic, thyrotropic, and corticotropic axes during development of Langshan and Arbor Acres chickens.
The data indicate that select genes may show parallel expression during certain periods of development, and that differences in BW and gene expression respond differently to nutrient intake in LS and AA chickens, may help improve the molecular breeding of chickens.
International Union of Pharmacology. XXXV. The Glucagon Receptor Family
Advances in the understanding of how these peptides exert their biological activities are discussed, with a focus on the biological actions and structural features of the cognate receptors.
Internalization and trafficking of the human and rat growth hormone‐releasing hormone receptor
This first study on the dynamics of the GHRH/GHRH‐R complexes using fluorescence imaging in a native environment compared to cell system models revealed that both receptor primary structure and concentration at the plasma membrane play important roles in internalization and trafficking of specific G‐protein‐coupled receptors (GPCR).
Single-nucleotide polymorphisms in the promoter of the growth hormone-releasing hormone receptor gene are associated with growth and reproduction traits in chickens.
Investigation of variations of the chicken GHRHR gene and their associations with growth and reproduction traits in 768 Beijing You chickens suggested that the three SNPs in the G HRHR promoter could be used as potential genetic markers to improve the growth and reproductive traits in chickens.


Molecular cloning and expression of a human anterior pituitary receptor for growth hormone-releasing hormone.
The cloning of a cDNA encoding a human GHRH-R from an acromegalic pituitary cDNA library is reported, which is a member of the secretin family of G-protein-coupled receptors and has 47%, 42%, 35%, and 28% identity with receptors for vasoactive intestinal peptide, secretin, calcitonin, and PTH, respectively.
Molecular cloning and expression of a pituitary-specific receptor for growth hormone-releasing hormone.
  • K. Mayo
  • Biology, Medicine
    Molecular endocrinology
  • 1992
A mechanism for cellular signaling by GHRH is defined and the opportunity to examine the role of the G HRH receptor in growth abnormalities that involve the GH axis is provided.
Structure, Function, and Regulation of the Pituitary Receptor for Growth Hormone Releasing Hormone
An important role for GHRH in body growth is suggested both by clinical studies with G HRH-producing tumors, and by animal studies withGHRH-expressing transgenes; in both cases growth hormone hypersecretion, pituitary somatotroph hyperplasia, and inappropriate growth are observed.
Ultrastructural evidence for endogenous growth hormone-releasing factor-like immunoreactivity in the monkey pituitary gland.
Growth hormone-releasing factor-like immunoreactivity was visualized in monkey pituitary gland by immunocytochemistry on ultrathin sections obtained by cryoultramicrotomy and cytological evidence for direct participation of growth hormone- releasing factor in the regulation of the somatotropic function was provided.
Prolactin receptor immunoreactivity in rat anterior pituitary.
The identification of receptors specific to PRL within different cells of the anterior pituitsary raises the interesting possibility that PRL may act via an autocrine or paracrine network in regulating anterior pituitary function.
Uptake and ultrastructural localization of a [125I] growth hormone releasing factor agonist in male rat pituitary gland: evidence for internalization.
The findings show the cellular specificity of GRF uptake by somatotrophs and the internalization process from the plasma membrane to the intracellular organelles (secretory granules, lysosomes, and nuclear membrane) and the appearance of label on the nuclear membrane suggests that GRF may have effects on the translocation of messenger RNA from nucleus to cytoplasm in somatosomes.
Growth hormone releasing factor, somatocrinin, releases pituitary growth hormone in vitro.
On the basis of the results reported here, hypothalamic GRF and tumor-derived GRF are qualitatively indistinguishable in their ability to stimulate the secretion of immunoreactive growth hormone in vitro.