Restoration of mature etiolated cucumber hypocotyl cell wall susceptibility to expansin by pretreatment with fungal pectinases and EGTA in vitro.

@article{Zhao2008RestorationOM,
  title={Restoration of mature etiolated cucumber hypocotyl cell wall susceptibility to expansin by pretreatment with fungal pectinases and EGTA in vitro.},
  author={Qingxin Zhao and Sheng An Yuan and Xin Wang and Yuling Zhang and Hong Mei Zhu and Changmei Lu},
  journal={Plant physiology},
  year={2008},
  volume={147 4},
  pages={1874-85}
}
Mature plant cell walls lose their ability to expand and become unresponsive to expansin. This phenomenon is believed to be due to cross-linking of hemicellulose, pectin, or phenolic groups in the wall. By screening various hydrolytic enzymes, we found that pretreatment of nongrowing, heat-inactivated, basal cucumber (Cucumis sativus) hypocotyls with pectin lyase (Pel1) from Aspergillus japonicus could restore reconstituted exogenous expansin-induced extension in mature cell walls in vitro… CONTINUE READING
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