Restoration of male fertileNicotiana by fusion of protoplasts derived from two different cytoplasmic male-sterile cybrids

  title={Restoration of male fertileNicotiana by fusion of protoplasts derived from two different cytoplasmic male-sterile cybrids},
  author={Dvora Aviv and Esra Galun},
  journal={Plant Molecular Biology},
  • D. Aviv, E. Galun
  • Published 1 November 1986
  • Biology, Medicine
  • Plant Molecular Biology
SummaryUsing the ‘donor-recipient’ protoplast-fusion technique, we have recently constructed several alloplasmic-like lines ofNicotiana in which the original cytoplasms (or part of them) of eitherN. tabacum orN. sylvestris were replaced respectively, either byN. undulata or byN. bigelovii cytoplasms. These cybridizations resulted in two kinds of cytoplasmic male-sterile (CMS) cybrid plants:N. tabacum withN. undulata-like cytoplasm andN. sylvestris withN. bigelovii-like cytoplasm. Fusion of… 
Restoration of normal stamen development and pollen formation by fusion of different cytoplasmic male-sterile cultivars of Nicotiana tabacum
Comparisons between stamen structure and mitochondrial DNA for eight fertile progeny from one cybrid plant led to the identification of several restriction fragments that appeared at enhanced levels in connection with normal stamen development.
A chimeric and truncated mitochondrial atpA gene is transcribed in alloplasmic cytoplasmic male-sterile tobacco with Nicotiana bigelovii mitochondria
It was found that the flower morphology typical of the Nta(big)S parental plants, as well as of the novel male-sterile cybrids, coincided with the presence of a chimeric atpA gene copy where an open reading frame of unknown origin was found to be linked in-frame to the 3′-end of a truncated atp a gene.
Somatic hybridization by microfusion of defined protoplast pairs in Nicotiana: morphological, genetic, and molecular characterization
The present study demonstrates that electrofusion of individually selected pairs of protoplasts (microfusion) can be used for the controlled somatic hybridization of higher plants.
Chondriome analysis in sexual progenies of Nicotiana cybrids
The chondriomes (the mitochondrial genomes) of sexual-progeny plants derived from eleven Nicotiana cybrids which resulted from donor-recipient protoplast fusions were studied; no variations were detected between the mtDNAs of plantsderived from a given second-generation plant.
Organelle analysis of symmetric and asymmetric hybrids between Lycopersicon peruvianum and Lycopersicon esculentum
The organelles of somatic hybrids obtained from symmetric and asymmetric fusions between the Lycopersicon species L. peruvianum and L. esculentum were analyzed by DNA hybridization methods, indicating that both organelle types sorted out independently.
CMS system inNicotiana: flower development, patterns of mitochondrial DNA and mitochondrial gene expression
The organization of the mitochondrial genome and expression of mitochondrial DNA was investigated in the three different alloplasmic CMS tobacco analogs and compared to the corresponding malefertile parentalNicotiana species, indicating that mitochondrial genome structure in Nicotiana is altered by substitution of the nuclear back-ground.
The transfer of cytoplasmic and nuclear genomes by somatic hybridisation.
Somatic hybridisation has an important role in complementing conventional breeding in providing the gross genetic structure of chloroplast, mitochondrial and nuclear genomes and can extend the transfer boundaries defined by sexual hybridisation.
Modifications of floral development in tobacco induced by fusion of protoplasts of different male-sterile cultivars
SummaryProtoplasts derived from different cytoplasmic male-sterile cultivars of Nicotiana tabacum were fused. Nearly 200 cybrid calli were regenerated into plants and their flower morphologies were
Absence of mitochondrial and chloroplast DNA recombinations in Brassica napus plants regenerated from protoplasts, protoplast fusions and anther culture
SummaryOver 400 Brassica napus plants regenerated from individual protoplasts, from protoplast fusions and from anther culture were analysed for chloroplast and mitochondrial genome rearrangements by
Brassica napus lines with rearranged Arabidopsis mitochondria display CMS and a range of developmental aberrations
The existence of numerous CMS lines with different mitochondrial patterns involving a species with a sequenced genome offers new opportunities to investigate the genetic regulation of CMS and its associated developmental perturbations.


Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana Sylvestris by fusion with X-irradiated N. tabacum protoplasts
It could be demonstrated that there was an independent segregation of chloroplast type and male fertility/sterility: several somatic cybrids were male fertile but tentoxin sensitive and others were tentoxin insensitive yet they were male sterile.
Cytoplasmic hybridization in Nicotiana: Mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions
The results indicate an independent assortment of chloroplasts and mitochondria from the heteroplastomic fusion products, which are similar to the respective patterns of the fertile and sterile fusion partner.
Somatic hybridization in Nicotiana: Segregation of organellar traits among hybrid and cybrid plants
The trait of cytoplasmic male sterility in tobacco is not an expression of the type of chloroplast genetic material.
Novel alloplasmic Nicotiana plants by “donor-recipient” protoplast fusion: cybrids having N. tabacum or N. sylvestris nuclear genomes and either or both plastomes and chondriomes from alien species
All fusion combinations resulted in at least some male-sterile cybrids, having either donor or recipient plastomes thus indicating that interspecific “donor-recipient” protoplast fusion is an efficient mean to produce plants with alloplasmic male sterility.
Morphological characteristics and chloroplast DNA distribution in different cytoplasmic parasexual hybrids of Nicotiana tabacum
Protoplast fusion makes possible the fusion of two different cytoplasms, allowing genetical analysis of cytoplasmic factors, and the study of the association of the modified flower type with the chloroplastic DNA isolated from the corresponding plant showed that cp DNA seems independent from the mechanism of cy Topalasmic male sterility in tobacco.
Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion
It is reported here, however, that the mitochondrial (mt) DNAs of cybrids are different from those of the parents and from the mixture of the two.
Progeny analysis of the interspecific somatic hybrids: Nicotiana tabacum (CMS) + Nicotiana sylvestris with respect to nuclear and chloroplast markers
The progeny of a fusion experiment involving N. sylvestris protoplastics and X-irradiated protoplasts of the cytoplasmic male sterile ‘Line 92’ were analyzed and male sterility was investigated.
Transfer of cytoplasmic male sterility by selection for streptomycin resistance after protoplast fusion in Nicotiana
SummaryCytoplasmic male sterility (CMS) in Nicotiana is located on mitochondrial DNA (mtDNA) on which no selectable mutation has been isolated. The possibility of co-transfer of CMS and a selectable
Interspecific chloroplast recombination in a Nicotiana somatic hybrid.
In the Pt14 chloroplast genome three N. tabacum and four N. plumbaginifolia parent specific restriction sites have been identified, indicating that the pt14 chloroplasts genome contains at least six recombination sites.
Novel composition of mitochondrial genomes in Petunia somatic hybrids derived from cytoplasmic male sterile and fertile plants
The mitochondrial genomes of petunia somatic hybrid plants, which were derived from the fusion of male fertile P. hybrida protoplasts with cytoplasmic male sterile P. parodii protoplastics, were analyzed by endonuclease restriction and Southern blot hybridization analyses and found no clear relationship between the mitochondrial DNA restriction patterns ofmale fertile somatic hybrids and those of the male fertile parent.