Restoration of colony-forming activity in osmotically stressed Escherichia coli by betaine.

@article{Roth1988RestorationOC,
  title={Restoration of colony-forming activity in osmotically stressed Escherichia coli by betaine.},
  author={William G. Roth and Mary P Leckie and D N Dietzler},
  journal={Applied and environmental microbiology},
  year={1988},
  volume={54 12},
  pages={3142-6}
}
Exposure of Escherichia coli to 0.8 M NaCl caused a rapid and large decrease in colony-forming activity. When such osmotically upshocked cells were exposed to betaine, colony-forming activity was restored. Betaine was able to restore colony-forming activity even when chloramphenicol inhibited protein synthesis. Thus, restoration was not the result of cell turnover. The cells were not killed by exposure to 0.8 M NaCl, because during exposure they accumulated ATP intracellularly. Betaine… CONTINUE READING

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