Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay: III. 72 coded chemicals.

  title={Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay: III. 72 coded chemicals.},
  author={Douglas Mcgregor and A Brown and Pamela Cattanach and Ian Edwards and Douglas McBride and Colin Riach and William J. Caspary},
  journal={Environmental and molecular mutagenesis},
  volume={12 1},
Seventy-two chemicals were tested for their mutagenic potential in the L5178Y tk+/- mouse lymphoma cell forward mutation assay, using procedures based upon those described by Clive and Spector (Mutat Res 44:269-278, 1975) and Clive et al. (Mutat Res 59:61-108, 1979). Cultures were exposed to the chemicals for 4 hr, then cultured for 2 days before plating in soft agar with or without trifluorothymidine (TFT), 3 micrograms/ml. The chemicals were tested at least twice. Significant responses were… 

The need for long-term treatment in the mouse lymphoma assay.

Combining results from the present and previous studies, 31 of 34 chromosome aberration-positive chemicals exhibited positive responses in the MLA, suggesting that the sensitivity of the MLA with 24 h treatment periods approaches that of the chromosome Aberration test.

Failure to adequately use positive control data leads to poor quality mouse lymphoma data assessments.

The mouse lymphoma L5178Y/TK1/–-3.7.2C mutagenesis assay (MLA) is widely used to identify chemicals that are capable of inducing mutational damage and would be expected to yield mutant frequencies far higher than those characteristic of other gene mutation assays.

Transformation of BALB/c-3T3 cells: II. Investigation of experimental parameters that influence detection of benzo[a]pyrene-induced transformation.

  • E. Matthews
  • Biology
    Environmental health perspectives
  • 1993
It is found that the induction of BaP-induced transformation behaved as though it was caused by a mutational event, and the magnitude of these responses were inversely correlated with the cytotoxicity of the treatment doses.

Further analysis of Ames-negative rodent carcinogens that are only genotoxic in mammalian cells in vitro at concentrations exceeding 1 mM, including retesting of compounds of concern.

It is concluded that the 10 mM upper limit in mammalian cell tests can be lowered without any loss of sensitivity in detecting genotoxic rodent carcinogens.

Oxidative Stress, Mutations and Chromosomal Aberrations Induced by In Vitro and In Vivo Exposure to Furan

It is shown that both furan and BDA present a weak (if any) mutagenic activity but are clear inducers of clastogenic damage and are strongly indicated as key lesions for chromosomal damage whereas oxidized base lesions are unlikely to play a critical role.

1,4-Dichlorobenzene-Induced Liver Tumors in the Mouse: Evaluation of the Role of Chlorohydroquinones

  • M. Muller
  • Biology, Chemistry
    Reviews on environmental health
  • 2002
Evidence that the biotransformation of 1,4-DCB to substituted hydroquinone species contributes to hepatic adenoma and carcinoma formation in mouse liver is considered, which has implications for human carcinogenesis.

The results of five coded compounds: genistein, metaproterenol, rotenone, p-anisidine and resorcinol tested in the pH 6.7 Syrian hamster embryo cell morphological transformation assay.

There was no simple correlation between in vitro genotoxicity, morphological transformation in SHE cells and rodent carcinogenicity and further research is required to accurately assess the role of the SHE assay in the carcinogenic risk assessment of new chemical entities.

The effects of boric acid on sister chromatid exchanges and chromosome aberrations in cultured human lymphocytes

BA showed a cytotoxic effect by decreasing the replication index (RI) and mitotic index (MI), and decreased the MI in a dose-dependent manner for the 24 h treatment period.

Trichlorfon induces spindle disturbances in V79 cells and aneuploidy in male mouse germ cells.

It is concluded that trichlorfon is a potent spindle poison in V79 cells and induces aneuploidy in mouse spermatocytes during meiosis.