Responses of mouse spinal neurones in culture to locally applied Phe-Met-Arg-Phe-NH2.


Embryonic mouse spinal cord neurones were maintained in a primary dissociated culture with a medium free from antibiotics. Intracellular recordings were made and the effects of local application of the neuropeptide FMRFamide were tested on selected neurones. Two types of response were seen. One response was depolarizing, with an accompanying decrease in… (More)