Response surface modeling and optimization of major medium variables for glycolipopeptide production

  title={Response surface modeling and optimization of major medium variables for glycolipopeptide production},
  author={Maurice George Ekpenyong and Sylvester Antai and Atim Asitok and Bassey Offiong Ekpo},
  journal={Biocatalysis and agricultural biotechnology},
Abstract An unusual strain of Pseudomonas aeruginosa readily produced a glycolipopeptide compound which reduced surface tension of fermentation broth from 71.31 dynes/cm to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/L. A response surface methodology (RSM) involving a 24 full-factorial central composite rotatable design was used to optimize major medium parameters to improve yield. Critical input variables in the design included waste-frying oil (X1-carbon source), urea (X2… Expand
Kinetic modeling and quasi-economic analysis of fermentative glycolipopeptide biosurfactant production in a medium co-optimized by statistical and neural network approaches
Results showed that bio-catalytic rates of lipase and urease reached exponential values within the first 12 h of fermentation leading to high specific rates of substrate consumption and bacterial growth, suggesting that glycolipopeptide biosynthesis may proceed through stationary phase transpeptidation of the Glycolipid base. Expand
Selection of Enterobacter cloacae Strain POPE6 for Fermentative Production of Extracellular Lipase on Palm Kernel Oil Processing Effluent
An Enterobacter cloacae strain was isolated from palm oil processing effluent and demonstrated ability to elaborate extracellular lipase on Tween 80-minimal medium using the rapid plate assay ofExpand
Statistical optimization of lipase production from Sphingobacterium sp. strain S2 and evaluation of enzymatic depolymerization of Poly(lactic acid) at mesophilic temperature
Abstract The current study focuses on the production of poly (lactic acid) (PLA) degrading enzyme from Sphingobacterium sp. strain S2 under optimized conditions. The important variables influencingExpand
Isolation, characterization, and optimization of biosurfactant production by an oil-degrading Acinetobacter junii B6 isolated from an Iranian oil excavation site
Abstract An enrichment program for hydrocarbon-utilizing bacteria capable of biosurfactant production isolated from soil samples was carried out on mineral salt medium supplemented with Iranian lightExpand
Purification and identification of surface active amphiphilic candidates produced by Geotrichum candidum MK880487 possessing antifungal property
Abstract The present study was held to optimize production, purify and identify biosurfactant from yeast strain Geotrichum candidum MK880487. Biosurfactant production was done on modified Hua mediumExpand
Water Soluble Fraction of Crude Oil Uncouples Protease Biosynthesis and Activity in Hydrocarbonoclastic Bacteria; Implications for Natural Attenuation
Three hydrocarbonoclastic bacteria isolated from crude oil-impacted mesotidal waters of the Niger Delta area of Nigeria, and identified, by 16S rRNA sequencing, as Vibrio fluvialis OWPB63, SerratiaExpand
The yeast-like fungus Aureobasidium thailandense LB01 produces a new biosurfactant using olive oil mill wastewater as an inducer.
The biosurfactant exhibited a better performance than the chemical surfactant sodium dodecyl sulfate (SDS) in oil dispersion assays, thus suggesting its potential application in bioremediation. Expand
Evaluation of the Effects of Nutritional and Environmental Parameters on Extracellular Protease Production by Stenotrophomonas acidaminiphila Strain BPE4
An aerobic mesophilic Gram-negative rod-shaped bacterium isolated from fermenting bean processing effluent (BPE) and identified as Stenotrophomonas acidaminiphila strain BPE4, produced anExpand
Bioprocess Optimization of Nutritional Parameters for Enhanced Anti-leukemic L-Asparaginase Production by Aspergillus candidus UCCM 00117: A Sequential Statistical Approach
The therapeutic enzyme exhibited 15-fold more cytotoxicity to myeloid leukemia cell line than to normal (HEK 238 T) cell and the manganese-dependent L-asparaginase from Aspergills candidus UCCM 00117 is recommended for further anticancer drug investigations. Expand
Statistical and Artificial Neural Network Approaches to Modeling and Optimization of Fermentation Conditions for Production of a Surface/Bioactive Glyco-lipo-peptide
Bioprocess conditions namely temperature, pH, agitation and duration were comparatively modeled using statistical and artificial neural network methods to predict and optimize product yield using the matrix of a central composite rotatable design (CCRD). Expand


Response surface analyses of rhamnolipid production by Pseudomonas aeruginosa strain with two response values
Response surface methodology and Box-Behnken experiment design were employed for optimization of biosurfactant shaking flask fermentation conditions of a Pseudomonas aeruginosa strain and the expected rhamnolipid yield increased dramatically. Expand
Utilization of response surface methodology to optimize the culture media for the production of rhamnolipids by Pseudomonas aeruginosa AT10
Pseudomonas aeruginosa AT10 produced a mixture of surface-active rhamnolipids when cultivated on mineral medium with waste free fatty acids as carbon source. The development of the production processExpand
Biosurfactant Production by Pseudomonas aeruginosa Grown in Residual Soybean Oil
Pseudomonas aeruginosa PACL strain, isolated from oil-contaminated soil taken from a lagoon, was used to investigate the efficiency and magnitude of biosurfactant production, using different wasteExpand
Economic Optimized Medium for Tensio-Active Agent Production by Candida sphaerica UCP0995 and Application in the Removal of Hydrophobic Contaminant from Sand
The new biosurfactant, denominated Lunasan, recovered 95% of motor oil adsorbed in a sand sample, thus showing great potential for use in bioremediation processes, especially in the petroleum industry. Expand
Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria
Optimization of the medium for biosurfactants production by probiotic bacteria (Lactococcus lactis 53 and Streptococcus thermophilus A) was carried out using response surface methodology. BothExpand
Response surface analysis and simulation as a tool for bioprocess design and optimization
Abstract In the present work, factorial design and response surface techniques were used in combination with modeling and simulation to design and optimize an industrial bioprocess. AlcoholicExpand
Selection of microorganisms for biosurfactant production using agroindustrial wastes
Manipueira comprises a potential alternative culture medium for biosurfactant production by the selected isolates and showed minimum surface tension of 28 mN/m (LB5a isolate) whereas for decanted manipueira the lowest value was 26 mN /m (isolate LB2a). Expand
A Pseudomonas aeruginosa Strain Ikw1 Produces an Unusual Polymeric Surface-Active Compound in Waste Frying Oil-Minimal Medium
A Gram-negative bacterium isolated from the sub-surface water of Ikang River, Niger Delta region, Nigeria produced an unusual biosurfactant in waste frying oil-minimal medium. Cultural andExpand
Low-cost fermentative medium for biosurfactant production by probiotic bacteria
Results obtained showed that supplemented cheese whey and molasses media can be used as a relatively inexpensive and economical alternative to synthetic media for biosurfactant production by probiotic bacteria, thus an attractive alternative as many of the potential applications for biosURfactants depend on whether they can be produced economically. Expand
Production of a Peptidoglycolipid Bioemulsifier by Pseudomonas aeruginosa Grown on Hydrocarbon
  • M. O. Ilori, D. Amund
  • Chemistry, Medicine
  • Zeitschrift fur Naturforschung. C, Journal of biosciences
  • 2001
A strain of Pseudomonas aeruginosa isolated from a polluted soil was found to produce an extracellular bioemulsifier when cultivated on hexadecane as sole carbon source. The emulsifier wasExpand