Response of myogenic determination factors to cessation and resumption of electrical activity in skeletal muscle: A possible role for myogenin in denervation supersensitivity

Abstract

1. We have prepared probes specific for the chicken myogenic determination genes MyoD, myogenin, myf5, and herculin and have investigated the expression of these genes in response to denervation and acute electrical stimulation in neonate chick muscle, using ribonuclease protection. 2. Upon denervation, herculin mRNA remains essentially unchanged, myf5 transcript levels approximately double, and MyoD message is up-regulated by two- to fivefold. In contrast, the message coding for myogenin, barely detectable in innervated muscle, rises dramatically (~200-fold) on the second day after nerve section; in this respect it resembles acetylcholine receptor (AChR)α-,γ-andδ-subunit mRNAs. Cohybridization experiments reveal that the increase in myogenin mRNA slightly precedes the rise in AChRα-subunit message. 3. Electrical stimulation of denervated muscle leads to an immediate decline in myogenin and AChRα-subunit mRNAs, with half-lives of less than an hour and approximately 4 hr, respectively; message stability measurements suggest that this is effected through a rapid shutdown of transcription. Messages coding for MyoD, myf5, and herculin decay much more slowly, as a result of slower turnover. 4. Previous experiments have indicated the involvement of ade novo induced (Tsay, H.-J., Neville, C. M., and Schmidt, J.,FEBS Lett. 274:69–72, 1990) autocatalytic (Neville, C. M., Schmidt, M., and Schmidt, J.,NeuroReport 2:655–657, 1991) transcription factor in the denervation-triggered up-regulation of AChRα-subunit expression; the denervation and electrical stimulation experiments reported here are compatible with the notion that myogenin is that factor. We have prepared probes specific for the chicken myogenic determination genes MyoD, myogenin, myf5, and herculin and have investigated the expression of these genes in response to denervation and acute electrical stimulation in neonate chick muscle, using ribonuclease protection. Upon denervation, herculin mRNA remains essentially unchanged, myf5 transcript levels approximately double, and MyoD message is up-regulated by two- to fivefold. In contrast, the message coding for myogenin, barely detectable in innervated muscle, rises dramatically (~200-fold) on the second day after nerve section; in this respect it resembles acetylcholine receptor (AChR)α-,γ-andδ-subunit mRNAs. Cohybridization experiments reveal that the increase in myogenin mRNA slightly precedes the rise in AChRα-subunit message. Electrical stimulation of denervated muscle leads to an immediate decline in myogenin and AChRα-subunit mRNAs, with half-lives of less than an hour and approximately 4 hr, respectively; message stability measurements suggest that this is effected through a rapid shutdown of transcription. Messages coding for MyoD, myf5, and herculin decay much more slowly, as a result of slower turnover. Previous experiments have indicated the involvement of ade novo induced (Tsay, H.-J., Neville, C. M., and Schmidt, J.,FEBS Lett. 274:69–72, 1990) autocatalytic (Neville, C. M., Schmidt, M., and Schmidt, J.,NeuroReport 2:655–657, 1991) transcription factor in the denervation-triggered up-regulation of AChRα-subunit expression; the denervation and electrical stimulation experiments reported here are compatible with the notion that myogenin is that factor.

DOI: 10.1007/BF00711232

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@article{Neville1992ResponseOM, title={Response of myogenic determination factors to cessation and resumption of electrical activity in skeletal muscle: A possible role for myogenin in denervation supersensitivity}, author={Craig M. Neville and Marlies Schmidt and Jakob Schmidt}, journal={Cellular and Molecular Neurobiology}, year={1992}, volume={12}, pages={511-527} }