Response of malignant and nonmalignant epidermal cell lines to tunicamycin

  title={Response of malignant and nonmalignant epidermal cell lines to tunicamycin},
  author={Miriam M. Brysk and Joanne Miller and Shu-Jen Chen and Peter C. Moller and Robert W. Stach},
  journal={Cell and Tissue Research},
SummaryExposure of fibroblasts to tunicamycin has been found to be cytotoxic for transformed cells, but not for nontransformed cells. With two mouse epidermal cell lines of common origin, we observe a contrary pattern: The malignant cells are more resistant to tunicamycin than their nonmalignant counterparts, as measured by growth and viability. With respect to the glycosylation of sugar precursors, the incorporation of mannose is more inhibited than that of glucosamine, while fucose is least… Expand


Effect of Tunicamycin on Cell Growth and Morphology of Nontransformed and Transformed Cell Lines
The effect of tunicamycin, an inhibitor of protein glycosylation mediated by dolichol, on the cell growth and morphology of several normal and transformed cell lines was studied. The low doses ofExpand
Selective cytotoxicity of tunicamycin for transformed cells
Results indicate that tunicamycin interferes with some cellular process critical for the survival of many transformed cells but not of non‐transformed cells, and may be therapeutically useful as an anti‐tumor agent to selectively kill certain types of malignant cells, while sparing non‐ transformed cells. Expand
Changes in surface properties of normal and transformed cells caused by tunicamycin, an inhibitor of protein glycosylation.
  • D. Duksin, P. Bornstein
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1977
Tunicamycin interferes with the insertion or function of one or more cell-surface glycoproteins, which could affect a number of cellular properties, including attachment, cell shape, and agglutinability by some lectins. Expand
Concanavalin A binding glycoproteins of epidermal cells.
Fresh tissue gave a radioactive profile similar to that for cultured differentiated cells, except for evidence of damage from the techniques used to separate the epidermis from the dermis (the damage being progressively more severe going from heat to ammonium chloride to trypsin separation). Expand
Effects of tunicamycin on B16 metastatic melanoma cell surface glycoproteins and blood-borne arrest and survival properties.
Tunicamycin-induced cell surface glycoprotein changes in B16 melanoma cells may interfere with tumor cell-host cell interactions that lead to arrest and survival of blood-borne malignant cells. Expand
Effect of tunicamycin on polarized membrane functions of an established kidney epithelial cell line.
The results suggest that the polarized sorting out of epithelial membrane proteins to apical and basolateral surfaces and their functions related to vectorial transepithelial fluid transport, monitored by dome formation, can persist unimpaired despite considerable underglycosylation of cellular glycoproteins and inhibition of cell proliferation. Expand
Inhibition of desmosome formation with tunicamycin and with lectin in corneal cell aggregates.
  • J. Overton
  • Biology, Medicine
  • Developmental biology
  • 1982
Evidence favors a stabilizing role for carbohydrates, acting at some point in the process of junction formation and leaves open the possibility that a “recognition” function may also be involved. Expand
Effect of tunicamycin on epidermal glycoprotein and glycosaminoglycan synthesis in vitro.
Tunicamycin may affect the synthesis and/or degradation of proteoglycan core proteins or the xylosyltransferase, and either N-asparagine-linked carbohydrates play little role in epidermal-cell adhesion or there is little turnover of these components inEpidermal adhesive structures such as desmosomes and hemidesmosomes during organ culture. Expand
Effects of Tunicamycin on the Proliferation, Adhesion and Differentiation of Chick Embryo Chondrocytes in Clonal Cell Cultures
The effects of tunicamycin, a specific inhibitor of lipid-carrier dependent glycosylation of protein, on the proliferation, adhesion, and differentiation of freshly liberated chondrocytes from chickExpand
Alterations in the distribution of glycoproteins in epithelial cells of murine colon after injection of tunicamycin
The feasibility of using tunicamycin in vivo and detection of the changes obtained by the silver methenamine method is demonstrated. Expand